Among specialized Bioculture companies in India, Team One Biotech focuses on robust consortia for tough industrial effluents. Email: sales@teamonebiotech.com Visit: www.teamonebiotech.com Discover More on YouTube – Watch our latest insights & innovations!- Connect with Us on LinkedIn – Stay updated with expert content & trends!

Case Studies Wastewater Treatment

Tannery Effluent Treatment with Aerobic System (Flow Rate: 950 KLD)

Key Problems in Handling Tannery Effluent:

High COD and BOD:
Organic matter from hides and chemicals used in tanning lead to very high COD and BOD, making biological treatment challenging and energy-intensive.

Salinity and TDS Issues:
Large quantities of salt used in hide preservation and soaking stages significantly raise the TDS, which affects microbial health and limits reuse potential, requiring high TDS wastewater solutions.

Toxicity from Sulfides and Solvents:
Effluents often carry toxic sulfides and various solvents or dyes that can inhibit biological activity, corrode equipment, and pose environmental hazards.

Reach out to us today to explore effective, sustainable solutions for managing tannery effluent and ensuring compliance with environmental standards.

Sludge Handling and Disposal:
The treatment of tannery wastewater generates hazardous sludge, especially with high chromium content, requiring safe disposal as per hazardous waste norms. This highlights the importance of chromium removal technology in modern industrial effluent treatment solutions.

A tannery ETP with a flow rate of 950 KLD had several basic issues in its aerobic treatment plant. The plant was equipped with a single aeration tank with one partition and a single clarifier. Due to multiple streams and heavy ammoniacal nitrogen, the plant was facing operational inefficiencies.

The Initial Approach

Our team conducted a comprehensive site evaluation to identify issues and areas of improvement. The following key challenges were observed:

High COD levels (>10,000 ppm) due to sulfides and other pollutants.
BOD levels exceeding discharge standards (>5,000 ppm).
Higher Ammoniacal Nitrogen Levels at the outlet of the aeration tank (<450 ppm).
Very high MLSS and poor settling.
High SRT.

T1B Aerobio: Enhancing Aerobic Treatment Performance

T1B Aerobio is a scientifically formulated microbial consortium designed to optimize aerobic wastewater treatment. Its robust strains have the ability to survive in high TDS wastewater solutions with halophilic strains, degrade ammoniacal nitrogen, sodium acetate, and other nutrients, and perform under variable pH & temperature. The bioculture also secretes various enzymes, regulates sludge levels, and supports sustainable ETP plant operations.

Execution

Plant Optimization:

Removal of excess sludge to improve oxygen transfer and microbial activity.
The recirculation rate was adjusted, and its stream was uniformly distributed in both sections.

Dosing Regime:
A 60-day dosing schedule was implemented:

Phase 1 (Days 1–30): High initial dose to establish microbial dominance.
Phase 2 (Days 31–60): Maintenance dose to sustain treatment efficiency.

Monitoring Parameters:

COD and BOD reduction.
Ammoniacal Nitrogen levels in both aeration tanks.
Sludge volume index (SVI) and microbial activity.

Observations

The addition of T1B Aerobio resulted in significant improvements in the aerobic system’s performance. Key observations are summarized below:

Parameters	Day 1	Day 15	Day 30	Day 45	Day 60
COD (ppm)	8500	7469	4331	1345	423
BOD (ppm)	3770	2891	1761	760	181
AN (ppm)	652	545	291	166	82

Results

COD Reduction: Achieved a 95.02% reduction by Day 60, ensuring compliance with regulatory standards.
BOD Reduction: Achieved a 95.19% reduction, meeting safe discharge norms.
AN Reduction: Achieved an 87.42% reduction by Day 60, aligning with discharge compliance.
Improved Sludge Quality: Enhanced microbial flocculation reduced SVI and improved sludge settling.
MLSS Levels: Controlled effectively, improving sludge settling quality.

Conclusion

The application of T1B Aerobio significantly enhanced the performance of the tannery’s aerobic treatment system. The plant achieved regulatory compliance for COD and BOD levels, stabilized DO levels, and improved sludge quality.

By leveraging trusted bioculture suppliers for industrial wastewater, partnering with reputed ETP and STP plant manufacturers in India, and integrating biocultures for wastewater treatment, Team One Biotech demonstrates leadership among industrial wastewater treatment companies in delivering sustainable, high-performance solutions for the industry.

industrial effluent treatment solutions ,tannery wastewater treatment ,high TDS wastewater solutions ,chromium removal technology, sustainable ETP plant operations, ETP and STP plant manufacturers in India ,bioculture suppliers for industrial wastewater ,industrial wastewater recycling and reuse leather industry ,effluent treatment biocultures for wastewater treatment, industrial wastewater treatment companies.

Among specialized Bioculture companies in India, Team One Biotech focuses on robust consortia for tough industrial effluents.

Email: sales@teamonebiotech.com

Visit: www.teamonebiotech.com

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Sumeet Sharma

Aug, Thu, 2025

Treating Petroleum refinery effluent with high Sulfide concentration

Case Studies Wastewater Treatment

Industrial Wastewater Treatment for Petroleum Refineries: High Sulfide Removal Using Biocultures

A reputed petroleum refinery approached us due to high concentration of sulfides in their effluents. They tried multiple solutions, including electroplating, RO, etc., but they were very cost-intensive. Also, they received multiple notices from the pollution control board and were paying heavy fines.

In such industries wastewater treatment methods like RO and chemical dosing prove unsustainable so we offered them biological wastewater treatment as an eco-friendly alternative.

To upgrade your facility’s efficiency with proven biological wastewater treatment methods, microbial solutions, and expert consultation, Contact Us.

ETP Details:

Parameter	Value
Flow (current)	450 KLD
Flow (design)	450 KLD
Type of process	Facultative
Capacity of UASB	1250 KL
Capacity of AT	450 KL
Retention Time	90.66 hours (combined)

Challenges:

Parameters (PPM)	Avg. Inlet	Avg. Outlet
COD	5500–9010	2200–4600
BOD	2500–5800	1300–3000
Sulfides	2000	2000
PAH	1250	680

Operational Challenges:

The primary treatment was working at 10% efficiency in terms of COD reduction
The biological treatment worked at an average of 50% efficiency in terms of COD reduction

They were struggling to control the higher Sulfide levels, and it was inducing shock loads as explained earlier. In this case, the Inadequate aeration in water treatment, systems contributed to sulfide accumulation, highlighting the need for advanced ETP water treatment process design and management.

Tackling Sulfides in ETPs:

To tackle sulfides in ETP, the presence of SOBs or sulfide-oxidising bacteria is a must. The SOBs oxidize sulfides into sulfates. To prevent sulfate accumulation, SRBs or sulfur-reducing bacteria are required; however, SRBs are only effective in anaerobic systems.

Issues with Process:

The main issue with the process was that there was no provision of a separate aeration tank before UASB, where sulfides need to be oxidized into sulfates. This gap in the industrial wastewater treatment design reduced system effectiveness and highlighted the importance of using effective biocultures for wastewater treatment.

The Approach:

The industry partnered with us to commission their UASB and aeration tank with increased capacity and restart the plant at its full capacity in terms of hydraulic load.

We adopted a 3D approach:

Research/Scrutiny:

Our team visited their facility to go through the process of the new ETP and to scrutinize the value-addition factors.

Analysis:

We analyzed the 3-month cumulative data of their ETP to see trends in the inlet-outlet parameters’ variations and the permutation combinations related to it.

Innovation:

After the research and analysis, our team curated customized products and their dosing schedules with formulation keeping in mind the plan of action to get the desired results.

This process is called bioaugmentation.
Our tailor-made microbial blends reflect Team One Biotech’s leadership among top biotech companies in India, offering scalable solutions based on site-specific microbial demand.

Desired Outcomes:

Reduction in Sulfide levels in the final outlet
Development of strong biology to withstand shock loads and prevent upsets
Making ETP more efficient regarding COD/BOD and PAH degradation
Reduction in FOG
Improved microbial culture for wastewater treatment effectiveness under both aerobic and anaerobic conditions

Execution:

Products Used:

T1B Aerobio: Our aerobic bioculture for wastewater treatment consists of blends of several strains SOBs and facultative microorganisms, usually bacteria, along with key trace elements on a complex inert media.
T1B Anaerobio: Our anaerobic bioculture blend consists of SRBs and other anaerobic microbes that effectively reduce sulfates into H2S and enhance COD/BOD control.

Plan of Action:

A tank of 300 KL before UASB was converted into an aerobic tank, and T1B Aerobio with SOBs was dosed for sulfide oxidation.
T1B Anaerobio was dosed in UASB for sulfate and COD reduction.
The addition of T1B Aerobio was also done in the aeration tank after UASB every day.

This strategic integration of wastewater treatment methods significantly boosted operational stability and treatment consistency.

Results:

Parameters (PPM)	Avg. Inlet	Avg. Outlet (Secondary Clarifier)
COD	5500–9010	900–1300
BOD	2500–5800	350–750
Sulfides	2000	180
PAH	1250	220

Before & After Bioaugmentation:

Performance Highlights:

The COD/BOD degrading efficiency increased from 50% to 83%
Sulfide reduction was achieved up to 91%
PAH was also getting degraded up to 82.4%
MLSS: MLVSS ratio was optimized
Biomass in the ASP system displayed great stability even during shock load situations
Methane gas production increased by 12%

These results demonstrate the superior impact of our biological treatment approach when combined with engineered aeration in water treatment design.

To upgrade your facility’s efficiency with proven wastewater treatment methods, microbial solutions, and expert consultation, Contact Us.

Email: sales@teamonebiotech.com

Visit: www.teamonebiotech.com

Discover More on YouTube – Watch our latest insights & innovations!-

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Sumeet Sharma

Jul, Tue, 2025

Case Studies Wastewater Treatment

Replacing Urea-DAP in a Textile Industry

Introduction:

We, at Team One Biotech LLP, help industries achieve sustainability goals with our biological wastewater treatment solutions, offering natural replacements for chemicals like urea-DAP and other nutrients.

In this success story, we are going to walk through the treatment efficiency and results achieved using SustainX for a leading textile company located at Dabhasa, GIDC, Gujarat. If you want to know more, feel free to contact us.

The industry happens to be one of the most compliant and efficient in terms of textile wastewater treatment, they had a very good ETP mechanism and trained staff. However, they had a huge consumption of UREA-DAP, leading to high OPEX, also they used to experience sudden spikes in Ammoniacal nitrogen values during winters.

ETP Flow chart:

Primary- Biological and Tertiary systems, with RO & MEE. The activated sludge process (ASP) has 3 aeration tanks in series and one anoxic tank before the aeration tanks.

Flow:	1500 m3/day
Inlet COD:	8,000 to 12,000 ppm
Inlet Ammoniacal Nitrogen:	280 to 320 ppm
COD outlet after biological treatment:	1800 to 2500 ppm
Ammoniacal Nitrogen after biological treatmeent	120 to 170 ppm	During Spike: 200-250 ppm
Urea – DAP consumption	800 kgs/day – 400 kgs/day respectively

Challenges:

Why traditional nutrients are inefficient in ETPS:

The commonly used urea and DAP are often overused in industrial treatment plants and they limit bioavailability for microbes. This results in poor nutrient uptake in biomass and limitations in COD and nitrogen removal causing ammonia spikes disturbing overall textile wastewater treatment systems.

1.The F/M ratio was low due to heavy consumption of UREA-DAP, leading to higher OPEX.

2.Sudden spike in ammoniacal nitrogen levels, especially in winters, due to non-consumption of ammonia from these fertilizers.

3.Saturation in COD is degrading efficiency.

The Scrutiny:

After a complete study of the plant through our WWTP evaluation form, on-site visits, and discussions with the EHS team, we concluded:

1.The F/M ratio gets lower if we stop urea-DAP addition.

2.The spike in ammoniacal nitrogen was due to the higher concentration of non-available nitrogen in the aeration tank, which was not absorbed by the biomass.

3.The indigenous microbial population was only performing at 60% efficiency in terms of COD reduction due to lower nutrient absorption despite a heavier dosage of UREA-DAP.

The Approach:

How T1B SustainX Works Better Than Urea-DAP:

SustainX is enriched with the naturally derived formulations comprising organic carbon and essential nutrients. It accelerates microbial metabolism and enhances organic waste degradation. Its unique formula helps eliminate side effects of urea and DAP and stabilizes the ammonical nitrogen. SustainX has proven benefits of optimising F/M ratio.

1. Product Selection:

Sustainx for textile wastewater treatment

T1B SustainX—The natural replacement of UREA—DAP.

Natural powdered blend with a well-balanced

C:N:P ratio, which will work in most scenarios.

Rich in organic carbon, bioavailable nitrogen, and phosphorus.

Contains natural trace elements and other nutrients essential for healthy biomass development.

Supports both aerobic and anaerobic microbes.

2.Dosing Schedule:

A dosing schedule was fixed for 60 days initially as follows:

Days	Quantity of T1B SustainX
Day 1 to Day 10	250 kgs/day
Day 11 to Day 20	200 kg/day
Day 21 to Day 30	200 kg/day
Day 31 to Day 60	180 kgs/day

3.The Execution:

The product was then dosed as per the efficiency of each aeration tank in series:

Tanks	AT-1	AT-2	AT-3
Capacity	2500 KL	1200 KL	1200 KL
% of total T1B sustain quantity added	60%	20%	20%

Results and discussions:

We observed a 94.5 % reduction in COD and a jump of 8% in efficiency from earlier values.
F/M ratio optimized to 0.234 , a rise of 64% was observed.
Use of MBR and the electricity to run the same was eliminated.
Improved the flow rate by 12% without compromising on the outlet parameters.

Email: sales@teamonebiotech.com

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Sumeet Sharma

Jun, Wed, 2025

Pond & Lake cleaner for successful biremediation

Case Studies Exports

Successful Pilot Trial of T1BTM Pond & Lake Cleaner for Lake Bioremediation in Riaba, Malabo

Client: Local Authorities, Riaba, Malabo
Conducted By: Team One Biotech LLP
Trial Period: 25th April – 5th May 2025
Technology Used: T1B™ Pond & Lake Cleaner – Microbial Bioremediation Solution

Background

Due to nutrient pollution (high phosphate and nitrate levels), the lake in Riaba, Malabo was experiencing notable ecological damage defined by:

Algal Bloom: High phosphate and nitrate levels of nutrient contamination cause too rapid algal development.
Sludge Accumulation: Two feet or more of organic sludge accumulated at the bottom of the water body.
Foul Odour: Strong anaerobic decomposition odour, especially during windy conditions.
Ecological Imbalance: Low dissolved oxygen (DO) levels affecting aquatic health.

Team One Biotech was engaged to pilot a microbial bioremediation approach using T1B™ Pond & Lake Cleaner, recognising the need for an environmentally sustainable solution.For more information or to collaborate on similar projects, Contact Us

Action Plan – Trial before Actual

Before initiating full-scale bioremediation of the lake, a pilot trial was strategically designed and executed to serve as a proof of concept. This small-scale trial aimed to:

Validate the effectiveness of T1B™ Pond & Lake Cleaner in a controlled environment. Understand product behaviour, microbial activity, and degradation patterns when exposed to real lake water conditions, including the presence of algae and organic sludge.
Simulate real-world conditions (aeration, nutrient load, microbial dose) in a manageable setting using a 200-litre drum.
Establish dosing protocols, response timelines, and key indicators for success (e.g., reduction in BOD, ammonia, and visible algal blooms).
Reduce the risk of large-scale deployment by gathering actionable data and visual proof of water improvement.
By conducting this pilot trial, Team One Biotech ensured a data-driven, confident, and customized approach to lake restoration. The outcomes from this trial form the foundation for scaling the treatment across the full lake body, with scientifically backed expectations for ecosystem rehabilitation and lake bioremediation aiming to restore ecological balance sustainably.

Project Objectives

1. Apply Advanced Bioremediation: Using T1B Pond & Lake Cleaner.

2. Restore Ecosystem Health: Cleanse and revive the water body.

3. Achieve Reductions In: Algal bloom – Nutrient levels (Nitrates, Phosphates, Ammonia) – Sludge volume – Foul odours

4. Enhance Water Quality: Improve clarity and DO.

5. Use Natural Microbial Technology: Target breakdown of pollutants and pathogens.

Methodology

Setup: A 200-litre drum filled with lake water containing visible algae and organic sludge.
Aeration: Continuous air supply to simulate natural mixing and oxygenation.
Dosing: 100g/day of T1B™ Pond & Lake Cleaner.
Observation Period: Daily monitoring of clarity, odour, algal reduction, and biological activity.

The Solution:

Natural microbial strains tailored for aquatic ecosystems.
Proprietary enzyme-plant extract blend.
Time-release formulation for sustained bioremediation.

Targets organic sludge, odours, and excess nutrients (ammonia, nitrates, phosphates).

Before vs After (10-Day Results)

Parameter	Before	After	Change
BOD (mg/L)	5.4	4.9	-9.26%
DO (mg/L)	5.2	4.8	-7.69%
Phosphate (mg/L)	1.0	1.0	No Change
Ammonia (mg/L)	0.5	0.3	-40.00%
pH	8.2	8.0	-2.44%

Key Observations After 10 Days

Algal Bloom: Noticeable reduction in algal density.
Water Clarity: Improved water clarity
Odour: significantly controlled
Biological Activity: Aerobic biological activity initiated

Conclusion & Recommendations

The trial successfully validated the potential of T1B Pond & Lake Cleaner in restoring lake water quality through natural and sustainable microbial bioremediation. The results showed measurable reductions in pollutants and improved clarity and ecological conditions within just 10 days.

Recommendation: Scale up the in-situ application across the lake using T1B Pond & Lake Cleaner, supported with significant aeration and regular monitoring.

Want Cleaner Water Bodies in Your Area?

Let’s make it happen — the natural way.

For collaboration, consultancy, or to implement this solution in your water bodies, please Contact Us.

???? Email: sales@teamonebiotech.com

???? Website: www.teamonebiotech.com

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Marshal

Jun, Tue, 2025

Case Studies Wastewater Treatment

Phosphate Removal in a Chemical Manufacturing Plant in Madhya Pradesh

A prominent chemical manufacturing unit situated in MP near Ratlam is our existing client to whom we provided technology to treat high COD and TDS effluent. They again approached us due to their experience working with us. They wanted to treat an effluent stream with high phosphate content upto 1500-2000 ppm. They asked us to use their old ETP, revive it , commission and make it efficient for phosphate treatment.

Looking to optimize your ETP for phosphate treatment, COD, or BOD removal?

Contact us to explore the right biological phosphorus and removal technologies for your industry!

1st Phase: Scrutiny

Our team of experts visited the factory to introspect and identify scope of improvements.

OLD ETP details:

The ETP had primary treatment, biological treatment (Anaerobic), and then a tertiary treatment.

Flow (current)	350 KLD
Type of process	UASB
No. of UASBR	1
Capacity of biological tank	950 KL

Parameters of the stream with Phosphate:

Parameters	Avg. Inlet parameters(PPM)
COD	4300
Phosphate Content	1500-1800
TDS	3000

2nd Phase : The Blueprint

After scrutiny and brainstorming with our R&D, we concluded to transform the old ETP apparatus into an EBPR unit, i.e., Enhanced Biological Phosphorus removal unit, which involves the introduction of PAOs (polyphosphate-accumulating bacteria) into the biological system along with physico-chemical treatment in primary and tertiary systems, respectively, of the old ETP.

ETP process optimization:

An efficient EBPR unit requires anaerobic as well as aerobic system, as in anaerobic the RbCODs get transferred into VFAs, which are then absorbed by PAOs for efficient phosphate uptake, which is dispersed during the anaerobic process. The PAOs then absorb the phosphate rapidly in the aerobic system. Hence, biomass with phosphate-absorbed PAOs is allowed to settle in the clarifier, and then WAS is removed.

In this scenario, the ETP had a UASB system, but no Aeration system, hence:

We utilized a spare tank of capacity 300 KL located next to USABR, and transformed it into an aeration tank by installing diffusers.
After our recommendation, the industry installed a 50 KL FRP clarifier after the sedimentation system.

Thus, we converted the old ETP into a facultative EBPR unit with integrated biological phosphorus removal capability.

3rd Phase : Technology and Execution

1. Selecting biocultures:

For UASB:

T1B Anaerobio:

The perfect solution for an Anaerobic system consists of robust bacteria that can efficiently work in anaerobic conditions, leveraging efficiency in terms of:

COD reduction
Biomass Generation
Methane Generation
F/M ratio optimization

Here, since our goal was phosphate treatment and reduction, we amalgamated PAOs as well, which made the product extremely effective to be used in the developed EBPR system.

For Aerobic Tank:

T1B Aerobio:

Equipped with highly robust and selective strains of bacteria which when combined with PAOs, made T1B Aerobio the best-suited weapons to remove phosphate levels, thereby increasing the efficiency of the EBPR unit.

2.Dosing:

Initially, we provided a dosing schedule for 60 days, in which 1st 30 days was loading dose, with a higher product quantity, and the second 30 days dose was maintenance dose, which was 1/4th of the loading dose.

Product	T1B Anaerobio	T1B Aerobio
Loading Dose	100 kgs	60 kgs
Maintenance dose	40 kgs	20 kgs
Point of addition	UASB	Aerobic Tank

3.Process optimization:

Our target was to achieve MLSS of 3500-4000 in the first 15 days. After that, the WAS was wasted at 15 KLD, and RAS was recirculated at 5 KLD.

Results:

After 60 days of implementation:

Parameters	Primary Outlet	UASB Outlet	Clarifier Outlet
COD	3900	1900	800
Phosphate	1300-1500	850-900	180
COD Reduction	10 %	~ 55 %	82 %
Phosphate reduction %	8-10%	~ 65 %	~85-90%

Conclusion

With the combined effect of T1B Anaerobio and T1B Aerobio bioculture and process optimization, the client achieved an 85-90 % reduction through the biological system, which further increased after tertiary system. This translated into:

Improved microbial activity and settleability.
Stable effluent quality, meeting compliance standards.
Biocultures are effective in phosphate removal.

This case demonstrates how biology-driven solutions, coupled with system know-how, can deliver tangible performance and cost benefits in industrial wastewater treatment.

Want similar results at your facility? Let’s talk!

Contact us nowto implement sustainable, biology-based solutions.

Email: sales@teamonebiotech.com

Visit: www.teamonebiotech.com

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Sumeet Sharma

May, Tue, 2025

Improving Oxygen Transfer Efficiency in Chemical ETP

Case Studies Wastewater Treatment

Improving Oxygen Transfer efficiency in a Chemical manufacturing plant

Background

A mid-size chemical manufacturing company situated in Madhya Pradesh was facing efficiency issues in improving oxygen transfer efficiency in its ETP, such as low efficiency, biomass suspension, and diffuser dysfunction. Despite maintaining a good overall diffused aeration system, their biomass was not developing, and MLVSS was very low.

As a result, the client incurred high CAPEXdue to unnecessary diffuser replacements and remained non-compliant with regulatory COD/BOD limits.Facing challenges in improving oxygen transfer efficiency and facing high energy costs? Let Team One Biotech help.

ETP details:

The industry had primary treatment, biological treatment, and then a tertiary treatment.

Flow (current)	750 KLD
Type of process	ASP
No. of aeration tanks	1
Capacity of aeration tanks	1150 KL

Challenges:

Parameters	Avg. Inlet parameters(PPM)	Avg. secondary system outlet parameters(PPM)
COD	18000	6000
BOD	8500	2800-3000
TDS	30000	2500

Problem Statement:

The client observed persistently low dissolved oxygen (DO) levels in the aeration tank despite extended blower run-times and increased air supply. This resulted in:

Sub-optimal biological treatment
Elevated energy costs
Occasional odor issues and inconsistent COD/BOD reduction

A preliminary diagnosis indicated biofilm accumulation and diffuser fouling, affecting fine bubble formation and limiting oxygen dispersion.

Our Approach

Team One Biotech initiated a comprehensive on-site audit including:

Diffuser Health Check

Inspected diffuser membranes for fouling
Identified scaling and microbial slimes affecting pore performance

Baseline Monitoring

DO levels across the tank: <1.5 mg/L
Specific Oxygen Uptake Rate (SOUR): <15 mg O₂/g VSS/hr
Blower energy use: ~65 kWh/day
OTE Baseline: Estimated OTE was 12%

Microbial Evaluation

Floc structure was loose, with filamentous dominance
Low settleability (SVI > 200)

To implement a cost-effective, eco-friendly bioremediation strategy that:

Enhances the degradation of formaldehyde and glutaraldehyde.
Restores biological treatment efficiency.
Achieves compliance with CPCB norms.

Solution

We proposed a 2-fold intervention:

1.Application of T1B Aerobio Bioculture

Dose: 10 ppm daily for 10 days, 8 ppm for next 10 days, and 5 ppm for next 10 days, then 3 ppm as maintenance every day.

Objective: Enrich native microbial diversity and improve biomass quality

2. Aeration System Optimization

Conducted sequential backflushing of diffusers
Realigned blower duty cycles with microbial demand using DO automation feedback

Monitored DO, pH, and ORP to ensure a stable environment.

Results:

After 60 days of implementation:

Parameters	Before intervention	After Intervention
DO in Aeration Tank	1.2 mg/L	2.8 mg/L
SOUR	1 3.6 mg O₂/g VSS/hr	22.3 mg O₂/g VSS/hr
SVI	210 mL/g	120 mL/g
COD Reduction	72%	87%
Blower Runtime	24 hrs/day	16 hrs/day
Energy Use	65 kWh/day	38 kWh/day
OTE	12 %	21.4 %

Conclusion

With the combined effect of T1B Aerobio bioculture and technical aeration optimization, the client achieved a 78.3% increase in oxygen transfer efficiency. This translated into:

Significant energy savings
Improved microbial activity and settleability
Stable effluent quality, meeting compliance standards

This case demonstrates how biology-driven solutions, coupled with system know-how, can deliver tangible performance and cost benefits in industrial wastewater treatment.

Ready to optimize your ETP performance? Connect with us today

Email: sales@teamonebiotech.com

Visit: www.teamonebiotech.com

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Sumeet Sharma

Apr, Sat, 2025

Seasonal Microbial Shifts Wastewater Treatment

Case Studies Wastewater Treatment

Bioculture for ETP- How a Textile Unit Stabilized ETP Performance with T1B Aerobio

Background

A mid-sized textile dyeing and processing unit in Gujarat struggled with recurrent seasonal drift in ETP and it’s biological performance. Contact us today to learn how T1B Aerobio can revolutionize your ETP’s performance and help you overcome seasonal challenges effectively.

Despite having a decent system design, they were plagued by:

Winter ammonia spikes
Monsoon washouts
Summer bulking
Transitional season shock-loads

These issues led to frequent compliance failures and operational stress.

T1B Aerobio-One Stop solution to seasonal drift:

T1B Aerobio – a blend of robust microbes especially bacteria , is the ultimate Thor’s hammer for seasonal cahllenges in any ETP. With a bank of 76+ different strains , T1B Aerobio was customized according to the challenges face by ETP in every season. It also consist various elements and enzymes which make it more efficient and a single solution for various challenges which no ordinary bioculture/microbial culture can deliver.

ETP details:

The industry had primary treatment, biological treatment, and then a tertiary treatment.

Flow	150 KLD
Type of process	ASP
No. of aeration tanks	3
Capacity of aeration tanks	650 KL each
Total RT	hours

Season-Wise Breakdown of Challenges & Solutions

????️ Winter Challenges (Dec–Feb)

Problems:

Nitrifier slowdown → High ammonia (>20 mg/L)
Low microbial activity → Increased F/M ratio
Reduced floc formation → Poor settling, turbid outlet

Solutions:

Pre-winter bioaugmentation with cold-active nitrifiers from T1B Aerobio Bioculture.
Increased MLVSS through controlled culture addition
Fine-tuned aeration to maintain DO around 3 mg/L
Reduced F/M by optimizing sludge wasting

Results:

Ammonia was reduced to <5 mg/L within 10 days. Sludge quality improved, and the outlet was consistently clear.

☀️ Summer Challenges (Apr–Jun)

Problems:

High temperatures → Oxygen depletion
DO <1.5 mg/L → Filamentous bulking

anti-filamentous dominant cultures through T1B Aerobio bioculture to suppress filaments
Boosted DO levels by adjusting blower run hours
Added foam control microbes to reduce surface scum and bulking

Results:

SVI normalized to 95–100 mL/g. Sludge settling and clarity improved; odor complaints dropped significantly.

????️ Monsoon Challenges (Jul–Sep)

Problems:

Heavy rainfall → Dilution & shock load
Surface runoff → Toxic load spikes
MLSS washed out → From 3500 to 1800 mg/L
Sudden pH shifts due to drainage ingress

Solutions:

Pre-monsoon culture buildup plan to fortify biomass using T1B Aerbio bioculture’s High-MLVSS variant
pH stabilization buffer introduced during heavy rains
Equalization tank aeration was increased to handle shock loads better

Results:

MLSS restored to 3100 mg/L within 7 days. COD removal stabilized at 90–92%. No emergency bypass required.

???? Transitional Season Challenges (Mar, Oct–Nov)

Problems:

Frequent influent variability due to batch changes
Occasional toxicity due to dyeing chemical overuse
Rapid shifts in temperature and pH → Microbial lag

Solutions:

Weekly parameter tracking and real-time microbial health checks
Targeted detoxifier blend dosing with Aerobio during chemical overload
Gradual culture build-up before full-load restart after holidays

Results:

The biological system became more resilient, absorbing fluctuations without crashing. No major deviations in any parameter

Parameter Snapshot Before vs After Aerbio Intervention

Parameter	Before	After T1B Aerobio
(Winter)	>20 mg/L	<5 mg/L
MLSS (Monsoon)	~1800 mg/L	~3100 mg/L
SVI (Summer)	>160 mL/g	90–100 mL/g
COD Removal	~78%	~92%
Outlet Clarity	Turbid frequently	Clear, consistent
Odor Complaints	Frequent	Almost Nil

Conclusion

Microbial performance doesn’t follow a flat line—it fluctuates with the weather. But with a season-wise microbial management plan, your ETP can remain compliant, efficient, and stress-free year-round.T1B’s Aerbio bioculture adapts where standard systems struggle—empowering your ETP to beat the seasonal drift, naturally.

Flow (current)	900 KLD
Type of process	ASP
No. of aeration tanks	2
Capacity of aeration tanks	3180 KL and 2840 KL

Parameters	Avg. Inlet parameters(PPM)	Avg. Outlet parameters(PPM)
COD	12000	1500
BOD	4500	880-500
TDS	4000	1200
Formaldehyde	200	145
Gluteraldehyde	210	182

ETP Details:

Operational Challenges:

Tackling Sulfides in ETPs:

The Approach:

Execution:

Results:

Before & After Bioaugmentation:

Performance Highlights:

Introduction:

ETP Flow chart:

Challenges:

Why traditional nutrients are inefficient in ETPS:

The Scrutiny:

The Approach:

How T1B SustainX Works Better Than Urea-DAP:

1. Product Selection:

2.Dosing Schedule:

3.The Execution:

Results and discussions:

Background

Action Plan – Trial before Actual

Project Objectives

Methodology

The Solution:

Before vs After (10-Day Results)

Key Observations After 10 Days

Conclusion & Recommendations

Want Cleaner Water Bodies in Your Area?

1st Phase: Scrutiny

OLD ETP details:

Parameters of the stream with Phosphate:

2nd Phase : The Blueprint

ETP process optimization:

3rd Phase : Technology and Execution

1. Selecting biocultures:

2.Dosing:

3.Process optimization:

Conclusion

Background

ETP details:

Challenges:

Problem Statement:

Our Approach

Solution

Results:

Conclusion

Background

T1B Aerobio-One Stop solution to seasonal drift:

ETP details:

Season-Wise Breakdown of Challenges & Solutions

Solutions:

Results:

☀️ Summer Challenges (Apr–Jun)

Problems:

Results:

????️ Monsoon Challenges (Jul–Sep)

Problems:

Solutions:

Results:

???? Transitional Season Challenges (Mar, Oct–Nov)

Problems:

Solutions:

Results:

Parameter Snapshot Before vs After Aerbio Intervention

Conclusion

Further Reading

Background

ETP details:

Challenges:

Problem Statement:

Objective:

Solution: Bioaugmentation-Based Bioremediation

Step 1: Selection of Microbial Culture/Bioculture

Step 2: Dosing Plan in Full-Scale ETP

Step 3: Acclimatization Phase (2 Days)

Results:

Benefits Observed

Conclusion