Sludge Bulking vs. Sludge Settling Ways to improve wastewater treatment in India
Sludge Bulking vs Settling: Biotech Companies in India

“Our MLSS is quite high, but we are not getting enough settling. “ or “Our biomass development is very good as our MLSS is high, but we have very little BOD/COD reduction”. these statements are often given by EHS managers. However, the concept of MLSS is completely misunderstood; it’s never the quantity of MLSS, it’s always the quality of MLSS. The settling of sludge and BOD reduction always correspond with how good the MLSS is, and not how much it is.

This blog intricately explains the difference between sludge bulking and sludge settling, and which factors are necessary to look out for.

Sludge Settling vs Sludge Bulking:

With the growing awareness of operational efficiency, several biotech companies in India are now addressing sludge bulking challenges through microbial innovation and advanced diagnostics.

Healthy Sludge Settling:

In a well-operating secondary clarifier, biomass flocs are compact, dense, and settle rapidly. The supernatant above appears clear, and the sludge blanket remains stable.

Sludge Bulking:

Here, the sludge appears fluffy, loose, and struggles to compact at the bottom. The supernatant turns turbid, and sludge blankets may rise or disperse.

ParameterHealthy SettlingSludge Bulking
SVI (Sludge Volume Index)80–120 mL/g>150 mL/g
Sludge appearanceDense, compact flocsLoose, filamentous flocs
SupernatantClearTurbid
Settling time20–30 mins>45 mins
CauseBalanced systemFilamentous overgrowth, F/M imbalance
Why Good MLSS ≠ Good Settling

Operators often celebrate high MLSS as a sign of strong microbial population. But MLSS is a mass reading-It doesn’t distinguish between healthy floc-formers and problem-causing filamentous organisms.

“ Think of it like body weight: Two individuals weigh the same, but one may be with lean muscle, the other with excessive fat.

In bulking scenarios, the bulk of MLSS is held together by filamentous bacteria-these long, thread-like organisms stretch out of flocs, creating open, web-like structures that trap water and resist compaction.

Reliable biocultures companies have been instrumental in developing floc-forming microbial strains specifically tailored for bulking control.

What Causes Sludge Bulking?
  1. Filamentous Bacteria Overgrowth

Common species: Type 021N, Sphaerotilus, Microthrix parvicella, Thiothrix

These bacteria thrive under specific conditions such as:

Low DO (<1.0 mg/l) – especially at floc centers.

High F/M ratios – excess food leads to dominance of fast-growing filaments

Nutrient Imbalance– N and P deficiency affect floc formation

Surfactants and FOG – common in food, dairy, and textile industries

Hydraulic surges – shock loading from upstream process

Leading microbial companies in India are providing industry-specific solutions for complex ETP issues, helping clients achieve consistent results in variable conditions.

 

  1. F/M Ratio Imbalance

Too much organic load relative to MLSS results in excessive microbial growth, and filamentous bacteria often outcompete floc-formers.

Ideal F/M ratio: 0.2-0.5 kg BOD/kg MLSS/day

Bulking is more likely when F/M > 0.6 or < 0.1, especially during inconsistent feed conditions.

  1. pH and Toxic Shocks

Sudden changes in pH (below 6.5 or above 8.5) , or toxic loads (solvents, phenols, metals) can kill floc-formers and allow filaments to dominate during regrowth. However, Solutions like those from Team One Biotech, a known player among bioculture for ETP STP plant manufacturers, are reshaping how industries manage MLSS health and sludge behavior.

 

Decoding SVI and other key Indicators

Sludge Volume Index (SVI) is the gold standard for assessing settleability.

  • SVI = ( Settled sludge volume in 30 mins, mL/L) / MLSS (g/L)
  • SVI < 100 = Good settling
  • SVI 120–150 → Early warning of bulking
  • SVI > 200 → Severe bulking

Other red flags:

  • Rising sludge in the clarifier
  • Scum layer formation
  • Poor TSS in final discharge
  • Varying DO and pH patterns in aeration tanks
Countermeasures- How to fix Bulking?

In addition to microbial solutions, industrial odor control systems  also play a pivotal role in overall ETP performance and workplace hygiene.

Short-Term Fixes:

  • Chlorination or Peracetic Acid Dosing: Targets filamentous bacteria selectively. Start with 0.5–1 ppm, monitor response.
  • Increase DO Levels: Maintain >2.0 mg/L throughout the aeration tank, especially in large tanks or tanks with dead zones.
  • Sludge Wasting: Reduce SRT (sludge retention time) to control filament growth. Remove excess MLSS.
  • Polymers in Clarifier: For emergency clarity issues, short-term use of cationic polymers can compact sludge.

Long-Term Solutions:

  • Nutrient Balancing: Maintain COD:N:P at approx. 100:5:1. Add urea or DAP if needed.
  • Equalization Tank: Smooth out hydraulic/organic loading rates to the aeration tank.
  • Bioculture Regeneration: Consider seeding with robust floc-forming consortia after bulking episodes.
  • Upgrade Aeration: Switch to fine-bubble diffused aeration systems to improve oxygen transfer.
  • Micronutrient Support: Trace metals like iron, cobalt, and molybdenum support healthy floc formers.

If you’re exploring biocultures for ETP plant manufacturers in India or need effective bacteria solutions for wastewater treatment, Team One Biotech offers proven blends tested across sectors.

Conclusion:

Remember one quote: What settles well, treats well. MLSS and BOD tell only one part of the story – settleability, floc health, and microbial balance complete the picture.

As experts and EHS leaders, we must look beyond the dashboard. A 3500 mg/L MLSS might impress, but if your sludge floats and supernatant clouds, your ETP is already sending you a warning.

Looking for a trusted waste water treatment company to resolve sludge settling problems? Contact Team One Biotech today for tailored solutions and microbial consultation.

📧 Email: sales@teamonebiotech.com

🌐 Visit: www.teamonebiotech.com

🔹 Discover More on YouTube – Watch our latest insights & innovations!-

🔹 Connect with Us on LinkedIn – Stay updated with expert content & trends!

Toxic Shockwaves Travel Through ETPs How to Deal
How Toxic Shockwaves Travel Through ETPs: A Deep Dive into Impact, Zone-Wise Failure, and Recovery

A sudden or abrupt change from regular mechanisms, schedules, habits, or play is detested everywhere, right from living to non-living beings and from nature to industries or the metropolis.  These sudden changes sometimes come with the signs of change that, if identified at the right time, either prevent or make one prepare. But not all thunders come up with lightning.

Here, as we talk about wastewater treatment in ETPs, shock loads remain one of the most common and feared issues.With the onset of shock loads or the sudden introduction of a toxic system with lethal compounds leads to complete disarray in the system, and the whole microbial population gets attacked and damaged and it a tough task to reboot it and get it back to its normal stage.

However, if we know how toxic shockwaves in ETP travel in different systems and what signs the system produces before and during the onset, we can empower us to control this unwanted phenomenon.👉 Need expert support in handling or preventing toxic shockwaves in ETP? Contact our team at TeamOne Biotech for consultation, solutions, and support.

Let’s explore the shockwave travel mechanisms, early signs of warning, zone-wise failure and how to recover.

What is Toxic Shock ?

A sudden short-terms ingress of physical or chemical conditions that disrupts routine mechanisms an d disrupts microbial populations.

The Culprits: Common Toxic Agents:

  • Heavy metals (e.g., Cr⁶⁺, Zn²⁺, Cu²⁺): Inhibit enzymes and damage membranes.
  • Phenols and aromatic solvents: Disrupt cell walls, denature proteins.
  • Quaternary ammonium compounds (QACs): Destroy microbial membranes.
  • Strong acids or alkalis: Denature enzymes and destroy extracellular polymeric substances (EPS).
  • High TDS or salts: Cause osmotic shock, dehydration of microbial cells.
  • Temperature spikes: Above 40°C can be lethal to most ETP microbes.

A high COD  is not always directly proportional to toxicity. Even in a batch with COD of 2000 ppm, a 50 ppm phenol will cause disruptions.

How do toxic shockwaves in ETP travel through each zone?

1.Anaerobic Zone:

The anaerobic digestors or UASB reactors break down organics into methane or carbon dioxide by acidogenic and methanogenic bacteria.

The Effect of Toxic Shock:

Methanogens are more prone to shock as they are highly sensitive to pH shifts, metals, and aromatic solvents. A toxic load here may: 

  • Kill methanogens outright, collapsing methane production.
  • Lead to accumulation of VFAs (volatile fatty acids), crashing the pH below 6.5.
  • Result in black sludge, gas bubbles, and floating scum layers.
Indicators:

  • Drop in biogas flow rate (if monitored).
  • pH drop in digester effluent.
  • Sulphide-like odor and gas toxicity.
  • Foaming or bubbling at inlet distribution zones.
Recovery Options :

  • Stop influent flow immediately
  • Neutralize VFAs to bring pH back to 7.2 to 7.6
  • Inoculate with fresh anaerobic bioculture.
  • Feed diluted influent after 3-5 days of stabilization
2.Anoxic Zone: The Invisible Impact Zone

The function of the anoxic zone is highly dependent on nitrifying and denitrifying bacteria. 

The Effect of Toxic Shock:

Denitrifiers are facultative—more robust than methanogens—but still impacted by solvents, surfactants, and metals.

  • Nitrate remains unreduced.
  • Partial reduction leads to nitrite accumulation, which is also toxic.
  • Disruption in redox balance halts nitrogen removal.
Indicators:

  • Rising NO₃⁻ or NO₂⁻ in secondary-treated water.
  • No bubbles or gas generation from the anoxic tank surface.
  • Slight odor of chlorine or nitric oxide due to nitrite oxidation.
  • No apparent foaming or color change—this failure is usually silent.
Recovery Options :

  • Supplement the carbon source ( eg, methanol or acetate ) to restart denitrification.
  • Check and adjust DO and ORP to stay below 0.3 mg/L and -100 to -300 mV, respectively.
  • Restart mixing gently—denitrification is sensitive to turbulence.
3.Aerobic Zone: 

Aerobic microbes (heterotrophs, nitrifiers) oxidize organics and nitrogen, producing CO₂, nitrate, and water.

The effect of Toxic Shock:

It is comparatively easier to identify shocks easily in Aerobic Zones:

  • Increase in soluble COD and turbidity due to Cell lysis.
  • Release of ammonia and phosphates into the water.
  • Poor settling followed by clarifier overflows due to the disintegration of flocs.
  • Pathogen population surge due to collapsed microbial competition.
Indicators:

  • Septic-like: conditions-black, greasy foam with foul smell.
  • A sharp increase in SVI.
  • Filamentous and Nocardia become prominent.
  • Sudden DO depletion even with aeration on.
Recovery:

  • Stop the influent
  • Maintain DO at 3-4 mg/l
  • Slowly start the hydraulic load with 25-30% for the first 5-6 days and then gradually increase.
  • Waste heavily to remove lysed or decayed biomass.
  • Start adding bioculture with robust and shock-tolerant bacteria.
System-Wide Effects Ripple effects:

Secondary Clarifier:

  • Overloaded with dispersed solids → turbid effluent.
  • Sludge blanket floats or rises.
  • Polymer usage increases for sludge settling.
Sludge Dewatering:

  • Decayed biomass becomes non-dewaterable.
  • Centrifuges and belt presses clog easily.
  • Sludge has high moisture content and low calorific value.
Tertiary Treatment:

  • UF/RO membranes foul rapidly with organic colloids.
  • Sand filters choke with fine, dispersed flocs.
  • Chemical dosing (PAC, alum) surges.
Recovery Timeline Framework

PhaseActionTypical Duration
Initial ArrestStop feeding, start aeration, dose buffers0–24 hours
StabilizationAdd bio-culture, monitor parameters1–3 days
Gradual LoadingResume with diluted or treated influent4–7 days
Full RecoveryReturn to design load with full microbial function7–15 days
Conclusion:

AN ETP is like a living ecosystem with uncertainties. If we can find our early warning signs, we can prevent the discrepancies arising due to toxic shock waves in ETP. Although it is a very tough scenario to tackle but if prevented in time, the chances of vulnerability become very less. 

👉 Facing recurring issues or need expert intervention? Reach out to TeamOne Biotech — your partners in effective wastewater treatment and process recovery.

📧 Email: sales@teamonebiotech.com

🌐 Visit: www.teamonebiotech.com

🔹 Discover More on YouTube – Watch our latest insights & innovations!

🔹 Connect with Us on LinkedIn – Stay updated with expert content & trends!

Phosphate Removal with Biocultures
The menace of Phosphate- How to deal with it using Biocultures?

Phosphates are one of the prominent water pollutants, as designated by the NGT in 2019. A Suo-motu cognizance was also taken by the NGT on detergents and especially phosphate accumulation in rivers and water bodies, causing toxic foam, algal deposition, and eutrophication. Phosphates also exert odour and color. Strict limits have been issued to control phosphate accumulation. However, at the wastewater treatment levels, phosphate removal is a bit tough job as it requires multiple stages, effective bioculture solutions, and technical expertise to do so. 

Although chemical and physical separation are essential, it is the bioculture that act as  game changers in phosphate reduction.👉 Want to know how to integrate biocultures in your treatment process? Contact Us to learn more.

Let’s explore the effectiveness and correct mechanism of phosphate removal using biocultures:

1.What are Phosphates?

Phosphates (PO₄³⁻)  are chemical compounds that contain phosphorus.  In industry, mostly chemical intermediates and food processing units have a high amount of phosphates.

2.Why is it a problem for ETP/STP?

  • Poor effluent quality: NGT and most pollution control boards are very stringent in the phosphate levels in the final outlet. If the criteria are not met, it may lead to a bad ESG report and even shutdowns.
  • Eutrophication: Phosphates promote excessive algal deposition and plant growth, leading to depletion of oxygen in receiving water bodies.
  • Effect on biological treatment: High phosphate content may disturb the biological/microbial population. It leads to even growth of filamentous bacteria, leading to sludge bulking, poor biomass settling, and compromising the efficiency of BOD/COD removal.
  • Increased Chemical Dosing costs: High phosphate = higher chemical use → higher sludge production → more dewatering and disposal costs.
  • Risk of Struvite Scaling:  in systems with high phosphate and ammonia, struvite (MgNH₄PO₄) may precipitate, causing scaling in pipes, pumps, and digestors, increasing OPEX and CAPEX.
3. Enhanced biological phosphorus removal (EBPR)

Enhanced biological phosphorus removal (EBPR) processes are designed to culture communities of microorganisms in MLSS that have the Phosphorus Treatment and Removal Technologies. It involves use of specific microbial strains and put in ETP as biocultures. The strains absorb phosphate and are PAOs (polyphosphate-accumulating organisms). These are likely to comprise a variety of bacterial subpopulations, including Acinetobacter, Rhodocyclus, and some morphologically identified coccus-shaped bacteria.

An ideal EBPR process starts with:

  • Anaerobic Zone: The PAOs are first subjected to an Anaerobic environment where Biodegradable COD is fermented into VFA (Volatile Fatty Acids), particularly acetate and propionate, which serve as food for PAOs. PAOs thus metabolize polyphosphate reserve and release phosphorus.
  • Aerobic Zone: In the Aerobic zone, the PAOs take up the released phosphates by multiplying and oxidizing carbon reserves built in the anaerobic phase. Here, WAS (Waste Activated Sludge ) and RAS (Return Activated Sludge) play a very important role.
Critical factors for the success of EBPR:

  • Influent Characteristics:  a minimum influent BOD:P ratio of 25:1 is necessary in order to provide adequate conditions for PAOs to thrive. Note that this ratio is applicable to the influent of the anaerobic phase of the EBPR process.
  • Integrity of the Anaerobic zone: Establishing and maintaining strict anaerobic conditions in the anaerobic zone is critical for PAOs to be able to consume VFAs and store carbon compounds. The presence of oxygen or nitrates will disrupt the process by placing PAOs at a competitive disadvantage with other bacterial populations.
  • Variability: Variability in flows can result in variable anaerobic and aerobic contact times, which can disrupt the process. Flow and load variability can also impact the influent BOD:P ratio. 
  • Dissolved Oxygen: Excessive dissolved oxygen should not travel back to the anaerobic zone hence, DO should be maintained between 0.5 to 1.0 mg/l at the end of the aeration zone.
Conclusion: 

Phosphate removal is different from conventional ETP operations. It requires the right microbes, technical know-how, and physical and chemical treatments. And when physical and chemical treatments are combined with biocultures, can enhance phosphate removal by up to 90%, and also improve microbial population management in wastewater.Ready to revolutionize your wastewater treatment system with biocultures? Contact Us today for customized solutions.

Inference: Phosphorus Treatment and Removal Technologies

To know more Call us @ 7769862121 or Mail : sales@teamonebiotech.com

T1B SustainX can solve the malnutrition of ETP! How and Why?

What you read in the book is always different in the real-world hook!! A quote so accurately framed that and can be applied in every professional aspect, including wastewater treatment. No matter how many SOPs or books we read, the ground reality is different, each ETP is different, each industrial effluent is different and one of the most overlooked challenges across these systems is the malnutrition of ETP, where the biological treatment process suffers due to imbalanced or inadequate nutrient supply.

In the world of industrial wastewater treatment, biological systems are the backbone of sustainable and cost-effective operations. But even the best industrial application of microorganisms can’t function without the right nutrients. And for the right nutrients, the same old C:N:P ratio is followed. And to make up this ratio, unfortunately, the conventional nutrient sources such as UREA-DAP, which are supposed to be used for agriculture, are often used in abundance in common effluent treatment plants (CETPs), which is itself a self-sabotage practice.This leads to a common but critical issue—malnutrition of ETP, where effluent treatment plants suffer from poor nutrient availability or imbalance despite excessive chemical input.

Now, readers must be wondering as to what the ideal solution should be for this, as for every nutrient requirement, we need separate chemicals, like for nitrogen, it’s UREA, for phosphorus, it’s DAP, etc.

Well, Team One Biotech has a solution to this universal problem as well. Introducing T1B SustainX- a natural blend of nutrients in powdered form. A 100% replacement of UREA, DAP, Phosphoric acid, and other conventional nutrients.

Team One Biotech’s T1B SustainX offers a smart, eco-friendly, and efficient alternative. Here’s why it’s time to reconsider your ETP nutrient strategy—and how SustainX provides a smart, eco-friendly, and efficient alternative. Contact Us to know how SustainX can transform your operations.

The problem of using fertilizers in Industries as the nutrient source:

Despite their widespread use, these fertilizers contribute to the malnutrition of ETP, disrupting microbial health and system performance.Industrial effluent is not same as soil where we can put the traditional fertilizers. Using such products may give results, but it has some side effects too such as:

  • Nutrient Spikes & Imbalances: Urea, DAP and other products tend to release ammonia and phosphorous very rapidly causing sudden spike in nutrient availability leading to shock induction in the microbes present.
  • Limited Bioavailability: A significant portion of these nutrients is lost through runoff or chemical interactions, offering poor uptake efficiency.
  • Sludge Bulking & Odors: Excess ammonia from urea or phosphorus from DAP can trigger undesirable side effects like bulking, foaming, and odor removal.
  • Eutrophication Risk: Residual nutrients in treated effluents can pollute water bodies, leading to algal blooms and ecological damage.
T1B SustainX: One stop Nutrition Solution

It is a revolutionary and advanced nutritional solutions consists of balanced C:N:P , which is bioavailable.

Key Benefits of SustainX:

  • Scientifically designed pre-balanced ratio — no need for DAP/urea
  • Boosts microbial growth under anaerobic process and stress
  • Enhances COD/BOD reduction
  • Reduces sludge and odor removal issues
  • Improves methane yield in anaerobic digestion of biomass
  • Improves sludge quality and settleability
  • Reduced operational upsets and foaming
  • Stable system performance over time
  • Reduces operational hassle of doing multiple products
Practical Replacement comparison:

ParameterDAP/Urea/Phosphoric AcidT1B SustainX (Science Power)
Nutrient AvailabilityImmediate (risk of spike)Gradual (consistent)
BioavailabilityMedium to lowHigh (organic complex)
Microbial DiversityLimited impactSignificant positive impact
Sludge ProductionModerate to highReduced and stabilized
Residual NutrientsHigh risk (eutrophication)Minimal residual nutrients
Environmental ImpactHigher pollution potentialEco-friendly and sustainable
T1B SustainX- Nutrient Profile

T1B SustainX is a one blend-multiple nutrient product that gives all the necessary nutrients in one dose:

  • Organic Carbon → Primary electron donor and carbon source for microbial growth and co-metabolic degradation.
  • Total Nitrogen → Essential for amino acids, nucleic acids, and enzyme production, driving biomass formation.
  • Phosphate → Supports ATP synthesis, genetic material integrity, and membrane stability.
  • Calcium → Strengthens cell walls, stabilizes enzymes, and enhances bioflocculation and sludge settling.
  • Magnesium → Key cofactor for ribosomes, ATP handling, and enzyme regulation.
  • Sulfur → Needed for sulfur-containing amino acids, coenzymes, and redox balance.
  • Essential Micronutrient Metal Cofactors + Organic Micronutrient Coenzyme Precursors + Nitrogenous Organic Monomers and Metabolic Precursors

It also includes essential micronutrient metal cofactors, organic precursors, and nitrogenous metabolic compounds to enrich biological sewage treatment plants.

Real-World Impact:

SustainX has proven effective across a wide range of industrial effluents, including:

  • Pharmaceutical & Chemical Wastewater
  • Distilleries, Dairies & Food Units
  • Textiles & Detergents
  • CETPs and STPs
  • Petroleum & Pesticide Industries

Whether dealing with high COD, high TDS, or complex toxic loads, SustainX addresses the root causes of malnutrition of ETP by offering a complete, bioavailable nutrient solution for stable, high-performance biological treatment.

Upgrade Your ETP Nutrition- A Smarter and Sustainable Way:

With increasing regulatory scrutiny and rising sustainability expectations, continuing with outdated nutrient practices is no longer viable. T1B SustainX empowers ETP operators to:

  • Reduce chemical dependency
  • Improve operational efficiency
  • Cut down secondary pollution
  • Foster robust microbial ecosystems

Learn more at www.teamonebiotech.com or reach out at sales@teamonebiotech.com/8855050575

🔹 Discover More on YouTube – Watch our latest insights & innovations!

🔹 Connect with Us on LinkedIn – Stay updated with expert content & trends!

Seasonal Microbial Shifts Wastewater Treatment
ETP Performance Drift Due to Seasonal Microbial Shifts
Why Weather Matters More Than You Think in Biological Wastewater Treatment

In the evolving field of biological wastewater treatment, the performance of an effluent treatment plant manufacturer-designed system is often expected to be consistent. Yet, seasonal changes bring unseen forces into play—namely, seasonal microbial shifts.

Yes, the weather outside does impact what’s happening inside your biological tank.

From anaerobic wastewater treatment facilities to residential wastewater treatment systems, the health and efficiency of your microbial workforce are key to sustainability. This article dives into how climate-driven microbial dynamics can cause performance drifts—and how proactive strategies can future-proof your system.

👉 Contact us to know how your ETP can be adapted for every season using customized biological solutions.

The Invisible Workforce Behind ETPs

The core of any biological treatment system is its microbial community in ETP. These microorganisms are responsible for breaking down organic pollutants, converting ammonia to nitrate, and ensuring compliance with regulatory discharge norms.

But just like any workforce, they too have their comfort zones.

Seasonal Microbial Shifts: More Than Just Temperature

Microbes are sensitive to environmental parameters such as:

  • Temperature: Metabolic rates slow down in colder months, especially for nitrifiers.
  • Dissolved Oxygen (DO): Oxygen solubility increases in winter but may be limited due to reduced blower performance or sludge blanket fluctuations.
  • pH & Nutrient Uptake: Seasonal variations in industrial discharge or rainfall can alter pH and nutrient availability, affecting microbial dynamics.
  • Hydraulic Load: Monsoon seasons often increase flow, diluting influent but stressing retention time and contact efficiency.

These subtle shifts can lead to a noticeable drift in performance—sometimes gradual, sometimes sudden.

Microbial Dynamics in Action

Here’s a simplified breakdown of how microbial populations can change across seasons:

  • Winter: Slow growth of nitrifiers (Nitrosomonas/Nitrobacter) → Ammonia carryover risk. Sludge settling improves due to reduced filamentous growth.
  • Summer: Faster BOD removal but potential filamentous bulking due to low DO at higher temps.
  • Monsoon: Washout of biomass and sudden influx of organics or toxins due to surface runoff or diluted effluent—impacting both MLSS in wastewater and treatment efficiency.
What Your Parameters Are Telling You (Seasonal Indicators)
ParameterIdeal RangeSeasonal Variation & What It Indicates
DO (mg/L)2.0 – 3.5<2.0 in summer = filamentous growth; >4.0 in winter with low activity = underperforming bugs
MLSS (mg/L)2500 – 4000Monsoon may dilute or wash out biomass, dropping MLSS suddenly
SVI (mL/g)80 – 120>150 in summer suggests bulking; <70 in winter may indicate compact sludge
F/M Ratio0.2 – 0.4Low in winter due to slow bug activity; high post-monsoon due to fresh organic load
Ammonia (mg/L)<5 (in outlet)Elevated in winter due to slow nitrification; low in summer if nitrifiers are active
pH6.8 – 7.5Rainfall or industrial shifts can push pH outside this range, affecting bug health

By tracking these parameters monthly or weekly, early warnings of microbial stress can be detected and acted upon proactively.

What Can Be Done?
  1. Seasonal Bioaugmentation
    Introducing robust microbial cultures tailored for low-temp or high-load conditions can bridge seasonal performance gaps.
  2. Data-Driven Monitoring
    Trends in DO, SVI, ammonia, and MLSS can forecast seasonal drifts before they become problematic.
  3. Adjust Operating Parameters
    Fine-tune aeration, sludge wasting, or HRT based on seasonal projections for improved biological nutrient removal.
  4. Preventive Culture Dosing
    Pre-dosing before seasonal change (e.g., winter onset or monsoon) can prepare the system for upcoming stress.
Final Thought

Weather is inevitable, but ETP failures are not. Understanding and anticipating microbial behavior shifts with seasons can be the difference between compliance and chaos.

Let’s stop blaming the bugs—and start working with them.

Have you observed microbial shift or performance drift in your ETP system? Let’s connect and explore how tailored microbial strategies can make your system season-proof.

📧 Email: sales@teamonebiotech.com

🌐 Visit: www.teamonebiotech.com

🔹 Discover More on YouTube – Watch our latest insights & innovations!-

🔹 Connect with Us on LinkedIn – Stay updated with expert content & trends!

Understanding Recalcitrant COD in Wastewater Treatment

Wastewater treatment plants (WWTPs) are designed to remove organic pollutants, typically measured as chemical oxygen demand (COD). However, not all COD is easily degradable. A significant portion, known as recalcitrant COD, poses a major challenge for treatment facilities due to its resistance to conventional biological treatment methods. If you’re looking for effective solutions to tackle recalcitrant COD in wastewater treatment, feel free to contact us.

What is Recalcitrant COD?

Recalcitrant COD consists of complex organic compounds that persist in the environment and do not break down easily by microbial activity. These compounds include industrial dyes, pesticides, phenols, pharmaceuticals, and certain synthetic chemicals. Their persistence in treated effluent can lead to environmental pollution and regulatory non-compliance. The removal of recalcitrant pollutants often requires integrating advanced oxidation processes with conventional wastewater treatment techniques to achieve highly efficient degradation.

Sources of Recalcitrant COD

Recalcitrant COD is commonly found in wastewater from industries such as:

  • Textile & Dyeing – Synthetic dyes and pigments (textile service)
  • Pharmaceuticals – Active drug ingredients (pharma service)
  • Petrochemicals – Hydrocarbons and solvents (chemical service)
  • Pulp & Paper – Lignin and chlorinated compounds (pulp & paper service)
  • Adhesives, Food, Dairy, Pesticides, and Rubber Industries – Contaminants from production and processing (adhesives service, food service, dairy service, pesticides service, rubber service)
Conclusion

Addressing recalcitrant COD is critical for achieving stringent waste water discharge standards and ensuring environmental sustainability. By integrating advanced oxidation processes with conventional biological treatment methods, industries can effectively reduce the environmental impact of their wastewater. Continuous research and innovation in water and wastewater treatment will pave the way for more highly efficient and cost-effective solutions.

For expert solutions in recalcitrant COD removal, consult with bioculture companies for wastewater treatment that provide customised culture and technical support tailored to industrial needs.

Are you dealing with recalcitrant COD in wastewater treatment? Contact us today to explore advanced treatment technologies tailored to your needs!

📧 Email: sales@teamonebiotech.com
🌐 Visit: www.teamonebiotech.com

🔹 Discover More on YouTube – Watch our latest insights & innovations!-

🔹 Connect with Us on LinkedIn – Stay updated with expert content & trends!

Implementation of SBR systems in CETP
Implementation of SBR System in a CETP with T1B Aerobio Bioculture
Introduction:

The Common Effluent Treatment Plant (CETP) situated in Rajasthan handles effluents from over 40 industries in the RIICO sector. Equipped with SBR system in CETP technology, the system faces difficulty in handling the load of Chemical Oxygen Demand (COD) above 2000 PPM, owing to discharges from textiles and chemicals. The SBR wastewater treatment system, with 4 biological tanks and 4 cycles a day, was struggling with its efficiency in terms of COD reduction, resulting in high outlet COD levels. This excess load was carried over to the Reverse Osmosis (RO) system, leading to membrane damage and increased operational expenses (OPEX).

To explore effective solutions for optimizing wastewater treatment and improving COD reduction efficiency, you can reach out to Team One Biotech

ETP details:

The industry had primary treatment, biological treatment, and then a tertiary treatment.

Flow (current)2 MLD
Type of processSBR
No. of aeration tanks4
Capacity of aeration tanks3 MLD each
Total cycles in 24 hrs4
Duration of fill and Aeration cycle1.5 hrs and 2.5 hrs respectively
Challenges: 
Parameters Avg. Inlet parameters(PPM)Avg. Outlet parameters(PPM)
COD3000800
BOD1800280-300
TDS30001200
Operational Challenges:
  • The primary treatment was working at only 5% efficiency in terms of COD reduction.
  • The entire SBR process was lagging in COD degradation efficiency and sustainability of Mixed Liquor Volatile Suspended Solids (MLVSS).
  • Carryover COD and unsettled biomass were traveling to RO membranes, causing severe damage.
The Approach:

The agency operating the CETP wastewater treatment plant approached us to solve these pressing issues.

We adopted a 3D approach:
  1. Research/Scrutiny:
    Our team visited their facility during the winter season as they faced many challenges. We scrutinized every aspect of the plant to assess the efficiency of each component.
  2. Analysis:
    We analyzed six months of historical data to identify trends in wastewater treatment parameters, including BOD removal efficiency, COD degradation, and total dissolved solids (TDS) reduction.
  3. Innovation:
    Based on our findings, we developed a bioaugmentation strategy by selecting customized products and designing a targeted dosing schedule.
Desired Outcomes:
  • Significant COD and BOD reduction, improving the efficiency of biological treatment systems.
  • Degradation of hard-to-treat industrial effluents and formation of stable biomass to handle shock loads.
  • Enhanced biomass settling, reducing carryover COD and preventing RO membrane damage.
Execution:

Our team selected two products :

T1B Aerobio Bioculture: This product consisted of a blend of microbes as bioculture selected as per our analysis to degrade the recalcitrant COD, and ensure sustainability in the SBR system in CETP. 

Plan of Action:
  1. We devised a 60-day dosing program, divided into two phases:
  • Day 1 to Day 30: Loading dose to accelerate microbial population growth and generate biomass.
  • Day 31 to Day 60: Maintenance Dose, to maintain the population of biomass generated.
2. Dosing Strategy:
  • Dosing was carried out in all 4 SBR aeration tanks during filling and aeration cycles to ensure optimum microbial activity.
Results:
ParametersInlet parametersTank 4 outlet parameters (ppm)
COD3000 ppm280-300 ppm
BOD1800 ppm60-82 ppm

diagram of before and after bioculture, SBR system in CETP
The implementation of bioaugmentation program by SBR system in CETP resulted in significant improvements in the performance of biological units in their WWTP:

✅ Achieved 90% COD and BOD reduction, compared to the previous 70% efficiency.
✅ Reduced CETP operational expenditure (OPEX) by 20%.
✅ Increased ETP capacity utilization to handle full hydraulic load.
✅ Improved biological process stability, making it more resilient to influents fluctuations.
✅ RO membrane health restored, reducing damage by 80%.

Conclusion:

The successful implementation of bioaugmentation with T1B Aerobio Bioculture led to an efficient, cost-effective, and sustainable wastewater treatment system. By enhancing COD degradation efficiency, reducing BOD levels, and improving biomass stability, the CETP wastewater treatment achieved outstanding results. This highlights the importance of biological wastewater treatment solutions in optimizing industrial effluent treatment processes.

 Discover how T1B Aerobio Bioculture can help you today!

Struggling with high COD levels in your wastewater treatment system? Contact us today to know more about how T1B Aerobio Bioculture can help you today!

📧 Email: sales@teamonebiotech.com

🌐 Visit: www.teamonebiotech.com

🔹 Discover More on YouTube – Watch our latest insights & innovations!-

🔹 Connect with Us on LinkedIn – Stay updated with expert content & trends!

Wastewater treatment plant for integrated textile industry
Effective Wastewater Treatment Plant for an Integrated Textile Industry in India
Introduction:

The Integrated Textile Industry is a leading cloth manufacturing company that involves denim production, cotton apparel manufacturing, and is also involved in the pulping of raw materials and paper manufacturing. With a strong commitment to environmental sustainability, the Integrated Textile Industry operates a waste water treatment plant (WWTP) at its textile manufacturing facility to treat the industrial effluent generated during its textile production processes.

However, the industry faced challenges in meeting the effluent discharge limits for certain pollutants, including the presence of components from reactive dyes, high chemical oxygen demand (COD), elevated biochemical oxygen demand (BOD), higher levels of color, and effluent temperature reaching up to 50°C. To address these challenges, the industry implemented a bioaugmentation program at its effluent treatment plant (ETP), which resulted in significant improvements in the wastewater treatment process and compliance with regulatory standards for industrial effluents.

Effluent Treatment Plant (ETP) Details:

The industry had primary treatment, biological treatment, and then a tertiary treatment.

Flow500-600 KLD
Type of processMBBR
No. of aeration tanks2 (in parallel)
Capacity of aeration tanks650 KL each
Total RT hours
Challenges:
ParametersInlet parameters Outlet parameters (Secondary System)
COD13,000 to 10000 8500 to 6800 
BOD4000 to 25002800 to 1650
Colour750 to 900 Hazen560 to 700 Hazen
  • The primary treatment system was working at 20-30% efficiency in terms of COD reduction.
  • The biological treatment was working at an average of 10-15% efficiency combined in terms of COD removal.
  • The system was struggling to effectively treat pollutants originating from reactive dyes and to reduce color contamination in the textile effluent.
  • The mixed liquor suspended solids (MLSS) were very low, and the microbial population in the biological treatment tanks could not develop due to the high wastewater temperature of 50°C.
  • The conventional MBBR waste water treatment plant was not efficient enough to consistently meet the stringent effluent discharge standards set by local environmental regulatory agencies.

As a result, the textile manufacturing company faced the risk of non-compliance, which could lead to regulatory fines, reputational damage, and environmental pollution.

The Bioaugmentation Approach:

The Integrated Textile Industry partnered with us to enhance the efficiency of their biological units. They had two aeration tanks in parallel, equipped with diffusers, handling a daily wastewater flow of 500-600 KLD.

Bioaugmentation is a biological wastewater treatment technique that involves adding specifically selected microorganisms, such as bacteria and enzymes, to improve the biological degradation of pollutants in a waste water treatment plant. The team conducted a comprehensive wastewater assessment to analyze the industrial effluent characteristics and the WWTP’s operational parameters, identifying the best bioaugmentation strategy for this textile effluent treatment plant.

Based on the assessment, a customized bioaugmentation program was designed and implemented. The microbial cultures were carefully selected to target organic pollutants, particularly contaminants from reactive dyes in the industrial effluent stream. Thermophilic bacteria were introduced to withstand high-temperature wastewater conditions and enhance the biological treatment process.

The bioaugmentation process was seamlessly integrated into the existing wastewater treatment process, and the performance of the WWTP was monitored over the next three months.

Improved Effluent Quality After Bioaugmentation:

Parameters

Inlet Parameters (ppm)

Outlet Parameters (After Bioaugmentation) (ppm)

COD (Chemical Oxygen Demand)13,000 to 10,0002,500 to 1,800
BOD (Biochemical Oxygen Demand)4,000 to 2,500800 to 650
Color (Hazen Units)750 to 900150 to 300
Results and Benefits of Bioaugmentation in Wastewater Treatment:

The implementation of the bioaugmentation program resulted in significant improvements in the performance of biological units at the wastewater treatment plant:

✅ Achieved around 80-84% reduction in COD & BOD levels in the treated industrial effluent.
✅ Attained 80-85% color removal efficiency, demonstrating visible improvement in effluent clarity.
✅ Enhanced microbial population growth in biological tanks, even at higher wastewater temperatures.
✅ The biological treatment system became more stable, reducing process fluctuations caused by influents variability.
✅ Increased plant reliability, ensuring consistent compliance with regulatory discharge limits.
✅ Reduced operational costs through optimized biological treatment efficiency.

The successful bioaugmentation application has helped the Integrated Textile Industry maintain regulatory compliance, improve wastewater treatment plant performance, and support their commitment to sustainable textile manufacturing.

🔹 Discover More on YouTube – Watch our latest insights & innovations!

🔹 Connect with Us on LinkedIn – Stay updated with expert content & trends!

Scan the code