Case Study High COD-High TDS effluent treatment and Elimination of MEE (1)
High COD-High TDS effluent treatment and Elimination of MEE
Background

With the head office located at Ankleshwar, this prominent chemical manufacturing company was spending heavily to treat its high COD effluent with high TDS. Their current ETP also did not have any biological system. The company connected us and gave us the challenge not only to treat the High COD effluent with TDS up to, but also to eliminate the use of MEE in order to save cost.

1st Phase: Scrutiny

Our team of experts visited the factory to introspect to identify the scope of improvements.

ETP details:

Flow (current) 400 KLD
Type of process Primary + Tertiary (no Biology)
No of spare tanks 4
Capacity of neutral tank 1 130 KL
Capacity of neutral tank 2 625 KL
Capacity of neutral tank 3 130 KL
Capacity of neutral tank 4 60 KL

Parameters:

Effluent Treated by MEE:

Parameters  Avg. Inlet parameters (PPM)
COD 30000-40000
TDS 150000-180000

Effluent Treated in ETP:

Parameters  Avg. Inlet parameters (PPM)
COD 30000-40000
TDS 20000
Current Scenario

ETP Process:

  • Batch process is followed till neutral effluent storage tank 1.
  • 60 HP pumps are present with a capacity of 80 KL/hr.
  • Both the streams are equalized, while the acidic stream is neutralized at the neutralization tank with lime (400-1500 kg).
  • The supernatant passes through the belt filter while the sludge is passed to the settling tank and then to CF1 and CF2
  • The supernatant is passed to 4 neutral effluent storage tanks one by one through gravity, with capacities 130 Kl, 625 KL, 130 KL, and 60 Kl respectively.
  • No Aeration in Neutral effluent storage tanks.

Wastewater treatment of COD BOD AND MEE Image (1).png

Challenges

Treating high COD was not the actual challenge, but treating the effluent with such high TDS up to 160000 ppm was near impossible, as:

  • Conventional biological wastewater treatment struggles at high Total Dissolved Solids (TDS) levels, especially above 10,000–20,000 ppm.
  • High TDS creates osmotic stress, impairs enzyme function, and can rupture microbial cell membranes.
  • At 160,000 ppm TDS, most microbial communities collapse, making biological COD degradation nearly impossible.
  • T1B Aerobio contains specialised, scientifically selected bio cultures that naturally survive and thrive under extreme salinity and high organic loading.
2nd Phase: The Blueprint

After scrutiny and brainstorming with our R&D, we concluded and agreed to transform the existing ETP setup into a fully-functional ASP-based ETP that can treat high COD of effluent with High TDS.

ETP process optimization:

Action Plan:

  • Conversion of current tanks into biological tanks for COD reduction
  • The Neutral storage tanks of 130 KL AND  625 KL were converted into a biological tank, for which fine bubble diffusers were installed.
  • The third neutral effluent storage tank of capacity 130 KL to be used as a clarifier with the provision of recirculation back to the tank of 625 KL through pumps
  • Elimination of MEE gradually
  • We started with 125 kld flow and eventually took the daily flow to 400 kld.
3rd Phase: Technology and Execution
  1. Selecting biocultures:

T1B Aerobio

Reduces aeration processing in Wastewater treatment. Improves functioning & efficiency of biological units in WTP. Useful in activated sludge process bioreactors & biodigesters

Team One Biotech’s unique microbial preparation “T1B Aerobio” consists of blends of several strains of microorganisms, usually bacteria. These organisms are isolated from nature and are not genetically altered in any way. They are selected based on accelerated reproduction rates and their ability to perform specific functions, such as good floc-forming capabilities, ability to degrade xenobiotic compounds, ability to survive in high TDS, degrade ammonia, sodium acetate, and other nutrients, ability to perform under variable pH & temperature, ability to secrete various enzymes, etc. 

T1B SustainX

  • Our product T1B SustainX is a 100 % replacement of UREA-DAP and other conventional nutrients. It consists: 
    • Organic CarbonPrimary electron donor and carbon source for microbial growth and co-metabolic degradation.
    • Total Nitrogen → Essential for amino acids, nucleic acids, and enzyme production, driving biomass formation.
  • Phosphate Supports ATP synthesis, genetic material integrity, and membrane stability.
  • Calcium Strengthens cell walls, stabilizes enzymes, and enhances bio flocculation and sludge settling.
  • Magnesium → Key cofactor for ribosomes, ATP handling, and enzyme regulation.
  • Sulfur → Needed for sulfur-containing amino acids, coenzymes, and redox balance.

Essential Micronutrient Metal Cofactors + Organic Micronutrient Coenzyme Precursors + Nitrogenous Organic Monomers and Metabolic Precursors

2. Process optimization:

Our target was to achieve MLSS of 3500-4000 in the first 15 days. After that, the WAS was wasted at 15 KLD, and RAS was recirculated at 5 KLD.

Results:

After 60 days of implementation:

Parameters  Primary Outlet) Neutral (aeration) tank 2 Outlet Clarifier Outlet
COD (PPM) 30000 6000                   >3500
COD Reduction ~ 80 % ~ 88 %
TDS (PPM) 160000  160000 160000

Wastewater treatment of COD BOD AND MEE Image.

MEE elimination:

In 90 days, the MEE was completely eliminated, thereby reducing overall wastewater treatment cost by 62%.

Conclusion

With the combined effect of T1B Aerobio bio culture and T1B SustainX – nutrient source and process optimization, the client achieved an 85-90 % COD reduction efficiency in ETP through the biological system, which further increased after the tertiary system. This translated into:

  • Improved microbial activity and settleability.
  • Stable effluent quality, meeting compliance standards.
  • Bio cultures are effective in high TDS effluents.

This case demonstrates how biology-driven solutions, coupled with system know-how, can deliver tangible performance and cost benefits in industrial wastewater treatment.

Looking to improve your ETP/STP efficiency with the right bioculture?
Talk to our experts at Team One Biotech for customised microbial solutions.

Contact+91 8855050575

Email:  sales@teamonebiotech.com

Visit: www.teamonebiotech.com

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Effective Wastewater Treatment in Speciality Agrochemical Industry
Effective Wastewater Treatment in Speciality Agrochemical Industry
Introduction:

The agrochemical industry generates a significant volume of industrial wastewater due to continuous cleaning, washing, and multiple manufacturing processes. An Indian multinational agrochemical company faced a major challenge in handling a high organic load generated from its production operations. One of its plants, located in Gujarat GIDC, manufactures multiple agrochemical products and was struggling to maintain wastewater parameters within Pollution Control Board (PCB) discharge norms. For expert solutions on managing industrial wastewater effectively, contact Team One Biotech today.

ETP Flow Chart:

The Effluent Treatment Plant (ETP) consists of Primary, Biological, and Tertiary systems, integrated with Reverse Osmosis (RO) and Multiple Effect Evaporator (MEE). The activated sludge process (ASP) includes three aeration tanks in series and one anoxic tank positioned before the aeration units to enhance biological treatment efficiency.

Flow Parameters:

Flow: 200 m3/day
Inlet COD: 14,000 to 17,000 ppm
Inlet Ammoniacal nitrogen: 280 to 320 ppm
COD outlet after biological treatment:   9000 to 12000 ppm
Ammoniacal Nitrogen after biological treatment 220 to 270 ppm

Challenges:
Despite maintaining high MLSS and MLVSS levels in all aeration tanks, the plant continued to record elevated COD, BOD, and Ammoniacal Nitrogen values, exceeding PCB discharge standards. The EHS department faced pressure to stabilize the biological process and meet environmental regulations. Some consultants even suggested incorporating a Membrane Bioreactor (MBR) after the ASP process, but it failed to deliver the expected COD and BOD reduction.

The Approach:
After a detailed evaluation using Team One Biotech LLP’s WWTP evaluation form, on-site 

inspection, and extensive discussion with the EHS team, it was concluded that the main issue was the absence of an effective microbial consortium in the biological treatment system. Additionally, multiple waste streams entering the ETP from various production campaigns further disturbed microbial stability. To address this, Team One Biotech performed a Wastewater Microbiome Analysis (WMA) and Effluent Treatability Study. These scientific evaluations helped determine the adaptability and growth of microbial cultures in the effluent, confirming that bioremediation could significantly reduce COD, BOD, and TAN levels.

Performance Evaluation:
The ETP performance was analyzed based on key parameters — Chemical Oxygen Demand (COD), Biological Oxygen Demand (BOD), Total Suspended Solids (TSS), pH, and Dissolved Oxygen (DO). Results revealed that with proper bioremediation and ETP optimization, the plant could achieve effluent quality within regulatory discharge limits.

Implementation Strategy:
The bioremediation program spanned over 60 days, where Team One Biotech bioaugmented all biological tanks, excluding the MBR. Interestingly, the MBR was later removed from the process, as the required output was achieved without it. The implementation was structured into three focused stages:

  • Plant Optimization: The influent flow rate was stabilized to prevent biological shock. Earlier, the flow fluctuated with production, which hampered microbial activity. It was converted to a continuous flow pattern for steady biological treatment performance.
  • T1B Aerobio Dosing: A 60-day dosing plan was executed with T1B Aerobio, a proprietary microbial formulation. The first four weeks included high dosing to increase microbial population density, followed by maintenance dosing for biomass stability.
  • Flow Rate Enhancement: The treatment capacity was gradually increased from 120 m³/day to 225 m³/day by the 60th day, maintaining consistent outlet quality.
Results and Discussions:


After 60 days, the plant achieved remarkable success: a 91% reduction in COD and 75% reduction in Total Ammoniacal Nitrogen (TAN). The COD levels decreased from ~15,000 ppm to ~500–450 ppm at the biological outlet. MLSS levels dropped from 18,000 ppm to 8,000–10,000 ppm, indicating improved biomass efficiency. The removal of the MBR system and its associated power consumption resulted in significant cost savings. Furthermore, the plant’s flow rate improved by 12%, and the RO membrane life increased due to reduced organic load. After a 3-month optimization phase, the use of RO was discontinued entirely, reflecting stable and sustainable ETP performance.

These outcomes demonstrate how Team One Biotech’s microbial bioremediation solutions effectively enhance industrial wastewater treatment efficiency and ensure compliance with PCB discharge norms. The project highlights how advanced biological treatment systems and ETP optimization strategies can reduce costs, improve environmental sustainability, and extend system life.

If you wish to improve your industrial wastewater treatment, achieve high COD and BOD reduction, and ensure sustainable ETP operations, connect with Team One Biotech LLP today. As one of the leading biotech companies in India, we provide a sustainable product range across multiple verticals, including probiotics for aquaculture, biofertilizers and plant growth promoters, eco-friendly cleaning solutions, animal probiotics, and on-site consultation for biocultures for ETP and STP.

Email:  sales@teamonebiotech.com

Visit: www.teamonebiotech.com

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Seasonal Microbial Shifts Wastewater Treatment
Bioculture for ETP- How a Textile Unit Stabilized ETP Performance with T1B Aerobio
 
Background

A mid-sized textile dyeing and processing unit in Gujarat struggled with recurrent seasonal drift in ETP and it’s biological performance. Contact us today to learn how T1B Aerobio can revolutionize your ETP’s performance and help you overcome seasonal challenges effectively.

Despite having a decent system design, they were plagued by:

  • Winter ammonia spikes
  • Monsoon washouts
  • Summer bulking
  • Transitional season shock-loads

These issues led to frequent compliance failures and operational stress.

T1B Aerobio-One Stop solution to seasonal drift:

T1B Aerobio – a blend of robust microbes especially bacteria , is the ultimate Thor’s hammer for seasonal cahllenges in any ETP. With a bank of 76+ different strains , T1B Aerobio was customized according to the challenges face by ETP in every season. It also consist various elements and enzymes which make it more efficient and a single solution for various challenges which no ordinary bioculture/microbial culture can deliver.

ETP details:

The industry had primary treatment, biological treatment, and then a tertiary treatment.

Flow150 KLD
Type of processASP
No. of aeration tanks
Capacity of aeration tanks650 KL each
Total RT hours
Season-Wise Breakdown of Challenges & Solutions

????️ Winter Challenges (Dec–Feb)

Problems:

  • Nitrifier slowdown → High ammonia (>20 mg/L)
  • Low microbial activity → Increased F/M ratio
  • Reduced floc formation → Poor settling, turbid outlet
Solutions:
  • Pre-winter bioaugmentation with cold-active nitrifiers from T1B Aerobio Bioculture.
  • Increased MLVSS through controlled culture addition
  • Fine-tuned aeration to maintain DO around 3 mg/L
  • Reduced F/M by optimizing sludge wasting
Results:

Ammonia was reduced to <5 mg/L within 10 days. Sludge quality improved, and the outlet was consistently clear.

☀️ Summer Challenges (Apr–Jun)
Problems:
  • High temperatures → Oxygen depletion
  • DO <1.5 mg/L → Filamentous bulking
  •  anti-filamentous dominant cultures through T1B Aerobio bioculture to suppress filaments
  • Boosted DO levels by adjusting blower run hours
  • Added foam control microbes to reduce surface scum and bulking
Results:

SVI normalized to 95–100 mL/g. Sludge settling and clarity improved; odor complaints dropped significantly.

????️ Monsoon Challenges (Jul–Sep)
Problems:
  • Heavy rainfall → Dilution & shock load
  • Surface runoff → Toxic load spikes
  • MLSS washed out → From 3500 to 1800 mg/L
  • Sudden pH shifts due to drainage ingress
Solutions:
  • Pre-monsoon culture buildup plan to fortify biomass using T1B Aerbio bioculture’s High-MLVSS variant
  • pH stabilization buffer introduced during heavy rains
  • Equalization tank aeration was increased to handle shock loads better
Results:

MLSS restored to 3100 mg/L within 7 days. COD removal stabilized at 90–92%. No emergency bypass required.

???? Transitional Season Challenges (Mar, Oct–Nov)
Problems:
  • Frequent influent variability due to batch changes
  • Occasional toxicity due to dyeing chemical overuse
  • Rapid shifts in temperature and pH → Microbial lag
Solutions:
  • Weekly parameter tracking and real-time microbial health checks
  • Targeted detoxifier blend dosing with Aerobio during chemical overload
  • Gradual culture build-up before full-load restart after holidays
Results:

The biological system became more resilient, absorbing fluctuations without crashing. No major deviations in any parameter

Parameter Snapshot Before vs After Aerbio Intervention
ParameterBeforeAfter T1B Aerobio
(Winter)>20 mg/L<5 mg/L
MLSS (Monsoon)~1800 mg/L~3100 mg/L
SVI (Summer)>160 mL/g90–100 mL/g
COD Removal~78%~92%
Outlet ClarityTurbid frequentlyClear, consistent
Odor ComplaintsFrequentAlmost Nil
Conclusion

Microbial performance doesn’t follow a flat line—it fluctuates with the weather. But with a season-wise microbial management plan, your ETP can remain compliant, efficient, and stress-free year-round.T1B’s Aerbio bioculture adapts where standard systems struggle—empowering your ETP to beat the seasonal drift, naturally.

Further Reading

To understand the science behind how microbial cultures enhance effluent treatment performance, explore our in-depth guide:
👉 What Are Biocultures for Wastewater Treatment — A Complete EHS Guide

This article explains the role of bioculture for ETP, the difference between aerobic and anaerobic bacteria, and how these biological solutions improve industrial wastewater treatment efficiency.

Contact us to implement a customized, season-wise microbial strategy with T1B Aerobio and keep your ETP biologically stable and compliant—year-round.

Email: sales@teamonebiotech.com

Visit: www.teamonebiotech.com

Discover More on YouTube – Watch our latest insights & innovations!-

Connect with Us on LinkedIn – Stay updated with expert content & trends!

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