Bioremediation in a Lake- a Tourist Hotspot

Introduction:

The lake is present in one of the popular cities of India and is itself a tourist hotspot. Hundreds of tourists visit the lake which is know for its scenic beauty and being one of the centres of attractions, the lake is surrounded by food parks, amusements, restaurants etc. However, during past few months the lake water suddenly started stinking and developed a lot of algae. Since, the lake was under the management of local municipal corporation. They employed physical cleaning along with installation of fountains of aeration but it was of no use. The odour was getting worst day by day thereby causing drop in the number of tourists and impacting the local tourism in terms of economy.

The municipal corporation then approached Team One Biotech to get the proper solution for the deteriorating conditions of the lake. We took a sample of the lake water and analysed it at our R&D lab.

Lake Details:

  • Capacity: 300 Million Litres
  • Area: 47 acres
  • Diameter: 30 sq meters
  • COD: 168 ppm
  • BOD: 87 ppm
  • Ammoniacal Nitrogen: 52 ppm
  • Algae: High Concentration

 

Findings and Challenges:

The COD, BOD and Ammoniacal Nitrogen were found to be high in concentration than the discharge limits. Also due to higher amount of AN, the algae growth was increasing rapidly made the water look greenish. Septic like odour was also present, though its source wasn’t initially clear, so we tried to find out any ingresses in the lake. We found that there was an ingress through an overflowing line of sewage nearby causing the lake water to smell and further deteriorating its quality.

The Approach:

After complete study of the lake through on-site visits, water sample analysis and  discussions with lake management and municipal corporation, we concluded that the sewage ingress and lack of management in terms of cleaning especially control on waste disposal.A Wastewater Microbiome Analysis (WMA) was carried out before providing a detailed techno-commercial offer. WMA reports of the sample revealed satisfactory growth and development of the microbes in the lake as well as gave us a complete analysis on the exact product and strategy to be employed for effective bioremediation.

Performance Evaluation:

The performance of our solution in lake was evaluated based on various parameters such as chemical oxygen demand (COD), biological oxygen demand (BOD), ammoniacal nitrogen, algae reduction, odor removal and color improvement . The results showed that the ETP was able to achieve the desired effluent quality as per regulatory standards.

The Entire bioremediation program ran for 6 months.

We designed a bioaugmentation and bioremediation programmme which was divided into three parts.

  1. T1B Lake Cleaner:  We planned a 180-day dosing schedule. It included strategic incorporation of T1B lake cleaner. Initially the first 4 weeks were crucial and therefore dosing was kept high to naturally increase the population of healthy microbes. Next 4 weeks and on the amount decreased & the primary agenda for dosing was to maintain a constant population of microbes in the system.
  2. Physical Cleaning and restrictions on waste disposal: Regular cleaning on lake water from non-biodegradable waste was setup. Strict restriction on waste disposal were also implemented with cooperation from Municipal corporation.
  3. Monthly monitoring of data and site inspection: Reports of the parameters were sent to us and was scrutinized by T1B to analyse the performance of the product and explore the areas of troubleshooting. On-site inspection was also initiated every fortnite to monitor odor and algae reduction.

Results and discussions:

  • We observed 75% reduction in COD and 76% reduction in  BOD and and 78.84 % reduction in TAN levels after 180 days.
  • Odour was completely eliminate.
  • Algae concentration became NIL.
  • Tourist footfall increased.

Connect with our experts:

+91 8855050575/sales@teamonebiotech.com

API CASE STUDY – Wastewater Treatment in multinational API pharmaceutical company

Introduction:

API and bulk drug manufacturers generate a considerable amount of wastewater through their manufacturing, washing, and cleaning processes. One Indian multinational pharmaceutical company encountered significant challenges in managing the high organic load produced from their manufacturing processes, specifically at a factory located in Gujarat GIDC, where they manufacture multiple APIs.

Details provided for Effluent Treatment Plant (ETP):

  • Primary, Biological and Tertiary systems, with RO & MEE.
  • The activated sludge process (ASP) has one anoxic tank and 3 aeration tanks in series.
  • Flow: Around 125 m3/day
  • Inlet COD: 18,000 to 22,000 ppm
  • COD outlet after biological treatment: 9000 to 17000 ppm

Findings and Challenges:

  • Despite the high amount of MLSS & MLVSS in all aeration tanks, COD, BOD, and Ammoniacal Nitrogen levels consistently exceeded discharge limits.
  • The EHS department of the industry faced pressure to maintain parameters according to PCB norms.
  • The implementation of an MBR after the ASP process, as recommended by some consultants, failed to produce satisfactory results.

The Approach:

The solution offered by Team One Biotech is a comprehensive strategy for improving the biological treatment of wastewater generated by API and bulk drug manufacturers. This strategy includes the use of robust microbial consortia and the implementation of various processes and technologies to optimize the performance of the Effluent Treatment Plant (ETP). Some specific solutions offered by Team One Biotech include:

1. Introduction of T1B Aerobio: Team One Biotech devised a 60-day dosing schedule that incorporated T1B Aerobio strategically. This microbial product was used to raise the population of healthy microbes naturally and sustain a consistent population within the system.

2. Increase in Flow rate: Team One Biotech aimed to achieve a flow rate of 225 m3/day by the 60th day. They gradually increased the flow rate on a weekly basis, starting from an initial flow rate of 120 m3/day. This increase in flow rate was achieved without compromising on the outlet parameters.

3. Wastewater Microbiome Analysis (WMA): Team One Biotech conducted a WMA to understand the current biological health of the plant. This analysis helped identify deficiencies in the appropriate biomass in the biological units and provided insights for developing effective solutions.

4. Implementation of MangEfficient microbes: Team One Biotech incorporated their MangEfficient microbes into the strategy for the API bulk drug Pharma unit. These microbes were used to enhance the treatment process and improve wastewater quality.

5. Elimination of shock loads: Team One Biotech eliminated shock loads by transitioning to a continuous flow rate, avoiding any abrupt or sudden changes in the biological process. This helped maintain consistent performance of the ETP.

6. Reduction in COD, ammonical nitrogen, and electricity usage: The solution provided by Team One Biotech resulted in more than 80% reduction in COD values, more than 70% reduction in ammonical nitrogen, and savings in terms of electricity usage.

Results:

  • We observed 85.8% reduction in COD and 75% reduction in TAN levels after 60 days and today the COD is in the range of 500 to 450 ppm in their biological outlet.
  • MLSS lowered from earlier 18000 ppm to around 8000 to 10,000 ppm in all the 3 tanks.
  • Use of MBR and the electricity to run the same, was eliminated.
  • Improved the flow rate by 12% without compromising on the outlet parameters.

Six Months Progress:

  • Eliminated shock loads by continuous feed
  • Carries out WMA to understand their current biological health
  • Introduced T1B Aerobic considering the plant conditions and their current process

After six months of monitoring, it’s evident that the outlet from the biological units consistently decreased despite fluctuating incoming loads. This consistent outcome suggests that our resilient microbial consortia effectively controlled all biological units.

Client Testimonial:

 “When we partnered with this T1B team, we were not really sure about the output, though we were confident that they would make some positive impact, but we didn’t anticipate the extent of the success we would achieve. We were struggling with low ETP performance & high cost in terms of electricity and solid handling not realizing the full potential of our Effluent Treatment Plant. Their team came in with a clear, well-thought-out strategy covering all aspects of the ETP in terms of performance, process and savings.

The results speak for themselves — more than 80% reduction in COD values, more than 70% reduction in ammonical nitrogen and saving in terms of electricity & solid handling. I wholeheartedly recommend their services to any business looking to revamp their digital marketing strategy and see real, quantifiable results.”

Are you facing similar challenges in industrial wastewater treatment? Explore the potential of bioremediation, connect with our technical experts today:

+91 8855050575 / sales@teamonebiotech.com

Bioremediation of Wastewater Treatment in Pharma Industries

“It’s an Illusion that the solution to pollution is dilution.”

This is India’s Century!! These words from S. Jayshankar echoed to every horizon of the planet and no single contradiction came, not from even the harshest critiques of India. This phrase is completely true in its sense and the pharmaceutical sector of India has made a tremendous contribution in making this phrase the “brahm vaakya”.

Current Scenario – Motion and Pollution

India ranks 3rd globally in pharmaceutical production, including generic drugs, OTC medicines, bulk drugs, vaccines, biosimilars, and biologics. It leads in supplying generic medicine with a 20% global share and is a major player in low-cost vaccines. India produces 60% of global vaccines, fulfilling up to 70% of WHO’s demand for Diphtheria, Tetanus, and Pertussis (DPT) and Bacillus Calmette–Guérin (BCG) vaccines, and 90% of WHO’s demand for the measles vaccine. Projections suggest the industry will reach $65 billion by 2024 and $132 billion by 2030.

Yet, this growth comes at a significant cost—pollution. India’s global achievements are marred by its 120th rank in the water quality index, with 70% of its water contaminated. Pharmaceutical industries are major contributors to this issue. Despite having wastewater treatment plants (WWTPs), many industries fail to meet compliance standards, leading to continued environmental challenges.

But then the question arises that if every pharmaceutical industry has a WWTP then why the compliances are not met?

Bioremediation – Exploring the Solutions

The challenges previously mentioned represent significant risks to the sustainability and operation of industries. However, much like a light at the end of a dark tunnel, Bioremediation emerges as a potential beacon of hope. When implemented effectively, it has the potential to revolutionize wastewater treatment. Nevertheless, there is a notable divide in opinions between industrialists and environmentalists regarding such claims. This skepticism is understandable, given that some companies present themselves as bioremediation companies without possessing the necessary expertise or foundational knowledge

So, we need to first understand the core of bioremediation which is the use of microorganisms. Now the readers will wonder that microorganisms are a vast group falling under Protista, do all of them work in ETP? So, this definition should be reframed as the use of the CORRECT microorganisms, especially selective bacteria to treat the wastewater.

Think of it like building a township: if the government hires without specifying the roles needed, it leads to chaos. But with careful selection—hiring engineers, doctors, and essential personnel—the township thrives. Similarly, bioremediation requires precise selection of microbial strains tailored to the specific needs of the treatment process for optimal results.

Challenges:

1. Tough to degrade Pollutants

Pharmaceutical effluents consist of some of the most tough to degrade compounds and pollutants such as carbamazepine and metformin. Compounds like these consist of NH and cyclic chain groups which makes them tough to degrade.

2. High COD and TDS

Due to the manufacture of drugs and medicines, few of the pharma effluent streams consist of very high COD and TDS up to 1 lakhs ppm which makes ETP operations difficult along with low sustainability of biomass, thereby leading to violation of compliances.

3. Shock Loads

Due to the manufacture of multiple products and constant changes in the constituents of effluent shock load situation is a common occurrence which leads to the sudden collapse of the ETP ecosystem even leading to serious conditions such as septic. Recovery is also very difficult in such  situations.

4. Poor MLSS:MLVSS Ratio

Due to the above-mentioned factors, natural biomass finds it difficult to develop to its optimum quantity which leads to poor MLSS:MLVSS ratio, which is one of the most important factors in the degradation of compounds in the secondary system.

5. Foul Odour 

Due to the presence of sulphides, mercaptans and other odour inducing compounds which mostly go undigested release a very foul and pungent odour which sometimes becomes dangerous for human health.

6. Hydraulic Load irregularities

Due to toxic and tough to degrade compounds, the EHS managers find it difficult to run the ETPs up to its maximum capacity which also sometimes affects the production.

7. Damage to RO/MEE membranes

Due to poor performances of ETPs and especially biological systems, the effluent carrying undigested pollutants eventually damages the membranes in the RO system.

8. Improper Plant design

With excessive pressure from pollution control boards and NGT it has become compulsory for industries having ETP, but many times low-experienced environment consultants suggest improper design thereby creating problems in waste-water treatment overall.

Solutions:

1. Tough to degrade Pollutants -Not so tough

Not so tough before bioremediation as compounds like metformin, carbamazepine and other aliphatic compounds can be easily degraded using the strain of bacteria which synthesizes enzymes like cytochrome P450s, peroxidases, etc. thereby degrading the myths of the afore-mentioned compounds being tough to degrade.

2. High efficiency in High COD and TDS

These high tides of COD and TDS can be easily sailed on the boat of bioremediation using paddles of microbes especially bacteria. Bacteria are not just the most primary, but one of the toughest organisms who possess the capacity to sustain and perform in high COD and TDS effluent, and degrading higher levels of COD closer to the permissible limits. Often a question is encountered whether bacteria membrane can sustain high amounts of salts or not. To be very specific there are a vast species of bacteria called halophiles which can easily sustain high amounts of TDS.

3. Shock Loads – The Myth Busted

Microbes with the shields of their enzymes can handle these shock waves of notorious effluents with shields of composure like rocks embracing high tides the sea shore. Efficiently working under shocking conditions and maintaining the efficiency of the biological system is one of the USPs of bioremediation. If a microbial consortium with a combination of selective bacteria is being assimilated into the system, then shock load management becomes very smooth enabling faster recoveries. This combination of strains makes sure that even if a different stream of effluent enters the system they act rapidly and maintain the degradation efficiency.

4. From Poor MLSS: MLVSS Ratio – to a Rich one

Bioremediation works towards upliftment of needy biological systems, especially those with poor MMLSS:MLVSS ratio. As explained earlier selective strain bacteria with the capacity to perform under certain conditions effectively maintain their cycle of LAG, LOG and Death phase which combined with efficient RAS (Return Activated Sludge) and WAS (Wate Activated Sludge) management improves the MLSS: MLVSS ratio.

5. Foul Odour – Pushed in past tense

Unfortunately, ETPs/STPs can’t opt for perfumes, but this distress has a savior called bioremediation. The sulphides, mercaptans and other odour-inducing compounds can be easily degraded by bioremediation thereby giving extremely high probability of odour removal.

 6. Hydraulic Load irregularities – From irregularities to punctualities.

Since the toxic and tough-to-degrade compounds easily be managed by bioremediation as explained before the EHS managers find it smooth to run the ETPs up to their maximum capacity which also sometimes can optimize the production.

7. Damage to RO/MEE membranes – Longevity ensured by bioremediation

Bioremediation do possess the tendency to tame the notoriously uncontrolled pollutants by either eliminating them or converting into simpler form like a constitution does to criminals by means of encounters or capital punishments.  Due to proper degradation of pollutants at microbial level, regulation of sludge, improved settling, improvement of MLSS:MLVSS the life span of RO membranes and MEE plant increases.

8. Improper Plant design-but a proper solution

Bioremediation is the best example for the concept- “Prosper in the Disaster”. Even if the plant design is improper, bioremediation with proper consultation on process management can manage the functionality of ETP thereby easing the pressures from CPCB/NGT.

Pharmaceutical industries do the noble work of manufacturing lifesaving drugs, products to fight diseases, ailments, traumas and epidemics. But when they need solutions to fight pollution or when they are in distressing time related to pollution agencies make them stand in court like trails and whole and sole blame them for the pollution despite of the efforts by the respective organizations to follow the norms. My message to the complete pharma diaspora is that you need not worry more when comes to pollution control. Bioremediation is there to give you a 360-degree solution that covers deliverance, compliance, sustainability, and cost-effectiveness.

To sum up, bioremediation is the Aspirin, or Nitroglycerine in wastewater treatment that relieves the ETPs from dangers of heavy pollution, plant failures. choking of membranes and non-compliances of the parameters.

Uncertain about how biocultures will handle wastewater treatment in your ETP/STP? Reach out to us today to discover more about our bioremediation technology from our wastewater specialists or schedule an on-site plant visit to assess your current processes in ETP/STP.

Explore our Wastewater Treatment Bioremediation Solutions or contact us at +91 885505075.

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